Apple pie @applepie@social.cleverlibre.org

Thrilled to share our lab's first paper in which we establish genome-wide CRISPR screening within a single mouse! We are so excited about the power of this platform to enable high-throughput genetic dissection of diverse phenomena within a living mammal.

https://www.cell.com/cell-genomics/fulltext/S2666-979X(22)00170-7

Managed to update a recycled talk for my unexpected 1pm #seminar during an incubation this morning and get two #nanopore library preps done and on the #GridION almost by 5pm this afternoon. I wish my average day was as productive.

Dorado v0.0.2 released!

- Faster basecalling
- 5mC calling with multi-GPU support
- Better performance on Apple M1 devices.

Stay tuned for a big release at NCM #nanoporeconf where I will be presenting BIG new Dorado features.

https://github.com/nanoporetech/dorado/releases/tag/v0.0.2

@gregggonsalves

Yes, vaccines, but let's not stop there. Air quality, masks, not exposing others when ill, thinking twice before gathering with large crowds indoors, these have worked for us before. We should not *again* count on pharma tools exclusively.

#influenza #AirQuality #immunocompromised #health

Mastodon: What you need to know for your security and privacy

If you’re leaving Twitter for Mastodon, here are some things you should know.

https://grahamcluley.com/mastodon-what-you-need-to-know-for-your-security-and-privacy/

Please reblog/boost if you think this would help folks you know who are new to Mastodon. (Likes are appreciated, but won't help spread the advice)

As a "fun" side project, I'm the lead author of the PopGen.jl suite of population genetics packages in the Julia programming language. It's ⚡FAST⚡ and 📒well documented 📒, written for easy onboarding. Have a look, try it out, let's chat!
https://biojulia.net/PopGen.jl/
https://github.com/biojulia/popgen.jl

How fast? Well, pairwise FST can be >600x faster than R::hierfstat on the same data. That's the difference between waiting 1 second and 10 minutes!

#popgen #julialang

A nice addition to the @ORCID_Org website to help integrate with Mastodon would be to support the use of the rel="me" attribute in a way that Mastodon users can use it to verify academic identities.

This would let links to ORCID's show in green with check marks on user profiles, thus quickly proving that the account is run by the person associated with that ORCID.

There's already an issue open, so go up vote if you'd like to see this supported

https://github.com/ORCID/ORCID-Source/issues/6668

👾 Tamagotchi! In #RStats? I've made a concept package that puts a cyberpet on your computer. Keep it alive in the R console! It persists between sessions.
📝 Blog: https://www.rostrum.blog/2022/11/13/tamrgo/
📦 Source: https://github.com/matt-dray/tamRgo

This is the Pink Fairy Armadillo.

They live in Argentina, have a furry bum to help them thermoregulate, and are around 3-4 inches long. It is said they can burrow through sand as fast as a fish can swim.

You're welcome.

A cell photographed through a microscope. Actin filaments (blue), DNA in the nucleus (red), and the Golgi apparatus (yellow) are shown. #CellBiology

Short thread on my poster from #biodata22 on detecting allelic imbalance at isoform-level and in single cells, work with Rob Patro, Noor Singh, Euphy Wu et al.

PDF here: https://www.dropbox.com/s/yjr0d4mndnozwmd/DATA_22_Love.pdf?dl=0

Reminder that my deep learning course at the University of Geneva is entirely available on-line. 1000+ slides, ~20h of screen-casts.

Full of examples in PyTorch

https://fleuret.org/dlc/

Let's start with the #FluorescenceFriday @ mstdn.science !!

Here you have "the explosion of life" during #cell division.

#microscopy #scicomm #organelles @cellcommlab

Protip:

When designing a user interface, imagine some old woman using it, say Margaret Hamilton, and she's clicking your app's buttons and saying to you, as old people do,

"Young whippersnapper, when I was your age, I sent 24 people to the ACTUAL MOON with my software in 4K of RAM and here I am clicking your button and it takes ten seconds to load a 50 megabyte video ad and then it crashes

I'm not even ANGRY with you, I'm just disappointed."

New training! Here I introduce the Seven Basic Tools of #Quality, with applications in LC-MS/MS. I hope you enjoy it! #Ishikawa #Proteomics

https://www.youtube.com/watch?v=IgoCLi6UVVE

Hi all 👋 One of our favorite things is making & sharing open source #genomics resources. Our latest is CRIPSRpedia – a free online textbook! Learn (& teach) about #CRISPR, from its role in nature to development of the genome editing tool, to applications in research 🔬, medicine 👩‍⚕️ & ag 🍓. Check it out! ➡️ crisprpedia.com

Oh and the awesome illustrations by Tami Tolpa are free to use for non-commercial purposes. 🎉

#introduction

A video associated with our recent paper on the role of MYO10 filopodia in breast cancer progression https://pubmed.ncbi.nlm.nih.gov/36283390/
Breast Cancer cells (magenta), collagen (green)

An #rstats #rladies workshop I gave earlier this year

Iterating well with {purrr} 🐱

now has the recording available 🎉

Thank you for having me, R-Ladies Rabat! 💜

https://www.pipinghotdata.com/talks/2022-06-08-iterating-well-with-purrr/