🔬First method-focused work from our lab is just out in Cell Reports Methods 🎉
Interested in multicolor SMLM? Check the open-access final version of Karoline Friedl's PhD work, with Sandrine Lévêque-Fort's lab and the Abbelight gang
https://www.cell.com/cell-reports-methods/fulltext/S2667-2375(23)00215-1
For all the folks starting their #PhD (or their master thesis) today - a reminder why your PhD advisor can solve a problem so "easily" ...
New @biorxivpreprint manuscript from my lab that sheds light on part of the dark genome, unannotated or poorly annotated genes in the genome. In it, we show that the highly conserved HEATR5 protein is a component of two biochemically distinct protein complexes.
If you are a white leftist and you talk “both parties are the same they are both capitalist” you will loose most of your black audience. I think it ought to be obvious that this isn’t because blackfolks love Democrats (in most cases, there is always someone being simple in any group) —no. it’s because the difference between the parties is material and obvious and these are unstable times.
When I hear such talk I wonder if the speaker is working on voter suppression.
@failedLyndonLaRouchite @MCDuncanLab mouth pipetting? 😅😱
@MCDuncanLab @failedLyndonLaRouchite @rspfau @lzvolk Good to know! [takes notes for future…]
@failedLyndonLaRouchite @MCDuncanLab @rspfau @lzvolk wow! Now I am imagining a lab of 10 people all intermittently shouting “10 uL!” in their best RP accent 🤣
Fellow professors, if you're teaching an introductory course in graduate school, set aside some class time early in the semester for students to discuss their transition to your program. You'll find that they're struggling far more than you probably realized. You won't be able to solve all their problems, but opening space for them to voice their issues is more powerful than you can imagine.
Best advice. Read the pipettor manual they are actually amazing resources.
Eg:
'To attain maximum accuracy, set volume must be approached from a higher value. If the desired value is lower than the previous volume, adjust the value to 1/3 turn above the required setting..."
Also these two pages:
@MCDuncanLab @rspfau @lzvolk as a postdoc I would love a how-to-pipet session. You always learn something new from someone else’s hard-won experience!
Absolutely! One thing I do now with everyone who joins my lab (even postdocs) is to do a how-to pipette session.
I think many of my early grad school failures were due to problems with pipetting.
I've started adding in, make sure to wear your glasses if you need them. Until I started needing reading glasses, I did not realize how much being able to see well is part of good pipetting. Now I realize that some ppl's struggles come from vision issues.
@rspfau @lzvolk @MCDuncanLab absolutely! There are so many things like this that are usually taught ad hoc and not systematically. How to stabilize your pipetting hand, how to aspirate liquid slowly and with control, how to minimize bubbles in viscous solutions, how to dip your pipet to a consistent depth every time, how to set your pipet volume (always turning the dial clockwise at the end). Small steps that add up to an intricate dance!
I strongly recommend this scholarly analysis of arguments over the status of the Neutral Theory
The sourcing is excellent, the logic is clear, and the analysis has a practical focus on facilitating science by achieving clarity (rather than adopting a partisan slant)
A minor disagreement: "The phrase ‘non-Darwinian evolution’ is a misnomer" (conclusion #6) is not justified in the text and is IMHO plainly wrong
Curious about the status on #qoto of the new opt-in full-text #search option to allow full-text search of posts. I've heard people chattering about it on other servers, but perhaps this Is this already enabled by default on qoto? Or will it be coming in the future?
I've been very impressed with the functionality of the instance and moderation decisions so far, so I'm sure the mods are on it (or already did it), but I couldn't find definitive info either way through some searching and the info page. Thanks! @freemo
Want to know what my research is about? Follow this thread 🧵 based on a 10min talk I've drawn for a meeting.
The talk was aimed at non-specialist space science colleagues (not the general public!). The slides were built up step by step, but I'm omitting this here & showing only the final graphs, less this becomes a 34-part thread. 11 is plenty enough!
So: "Understanding Winds of Massive Stars Using High Mass X-ray Binaries"
#astrodon #XraysAreTheBestRays #SciArt #scicomm #VicisAstro
1/11
@fisher_ke wow that is a silent epidemic! 😬 I’m pretty sure I exacerbated some ulnar nerve issues earlier this year with pipetting. Gotta be vigilant!
@MCDuncanLab new fear unlocked re: slipped disk! I also really like the mise en place idea. I could definitely benefit from organizing my space more.
Cell biologist and biophysicist studying evolutionary cell biology.
I'm interested in how amoebae divide, especially relatives of the "brain-eating amoeba"
I study this with microscopy, image analysis, and comparative genomics.
Postdoc at UMass Amherst Biology, PhD in Biophysics from Stanford.
I also love jazz and nature photography!
searchable