The very first time I did this, as an undergrad intern, I made 70% H20 instead of 70% ethanol...

"No one excels at a new experiment on their first try. When I was first taught how to extract plasmid DNA from bacteria, arguably one of the simplest protocols in my field, I think I messed up every possible step — there are ten — at least once."

https://www.nature.com/articles/d41586-023-02876-z?utm_medium=Social&utm_campaign=nature&utm_source=Twitter#Echobox=1694509194