Reading up on dyes, I see that the widely-used Alexa 594 performs poorly in #2Photon #imaging above about 850 nm.
We want a dye that works well at 1040 nm. According to this nice @biophysj paper, it looks to me like A546 would be a good choice, at least twice as good as A594. Do you have a favourite?
#fluorescence #cellbiology #neuroscience
https://www.sciencedirect.com/science/article/pii/S000634951200063X
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@andrewplested @biophysj I always preferred Atto 594 for 2P work. Much brighter than the Alexa. For longer wavelengths rhodamine b might be OK.
@ChrisWilms23 Thanks Chris!! This is a great tip.