Kidney epithelial #Tubuloid

CD24+ epithelial progenitor-derived
4-phase protocol, Matrigel +Wnt3a/RSPO1, grow > 6 mo
CRISPR modeling #ADPKD, Cyst size responsive to #Tolvaptan

Super-impressive single-cell or single-nuclei RNAseq analyses to dissect the spatiotemporal molecular/cellular dynamics of this cool renal tubuloid, laying a critical foundation to enhance its clinical utilities👍

Wonderful #Lumenogenesis of CD24+ epithelial progenitor-derived nephrospheres, day 7 in Matrigel🤠

Dr. Rafael Kramann lab @NatureGenet 2022 @jackxuyx @KuppeChristoph @rkramann
https://www.nature.com/articles/s41588-022-01202-z