Tools

"For a CRISPR guide RNA (gRNA) with a specific target but activity at known "off-target" sequences, we present a method to screen hundreds of thousands of gRNA variants with short, randomized 5' nucleotide extensions near its DNA-targeting segment"

#CRISPR #Preprint

https://www.biorxiv.org/content/10.1101/2023.01.11.523593v1