Harnessing anti-inflammatory potential of IDO.
Exciting new approach by @keselowsky, @GregHudalla using IDO therapeutically as an anti-inflammatory . (Short 🧵 1/5)
Suppression of local inflammation via galectin-anchored indoleamine 2,3-dioxygenase
Key advance:
Generating an IDO-Gal3 fusion protein.
Gal3 binds to fly and in the extracellular matrix (ECM) of tissues, anchoring IDO in place at the site of inflammation - very clever IMHO. (2/5)
IDO-Gal3 reduces the severity of symptoms in mice with established osteoarthritis.
They also show anti-inflammatory effects in skin inflammation and that IDO-Gal3 averts and inhibits periodontal disease progression. (4/5)
This study is exciting because it uses a direct intervention, using a recombinant anchored enzyme, to change nutrient levels within a local micro environment.
What enzyme would you like to use therapeutically?
And into what micro environment would you like to anchor it?
/end
Using a mechanical overloading osteoarthritis mouse model, mice were treated weekly with intra-articular IDO-Gal3 injections.
You can clearly see the retention of Gal3-IDO in the joint. (3/5)