Just reading this very interesting paper on how many #replicates are needed for appropriate analysis of bulk #RNAseq data and comparing tools for DE gene expression.

#bioinformatics #article #goodread

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878611/

From the abstract

With three biological replicates, nine of the 11 tools evaluated
found only 20%–40% of the significantly differentially expressed (SDE) genes identified with the full set of 42 clean replicates.
This rises to >85% for the subset of SDE genes changing in expression by more than fourfold. To achieve >85% for all SDE genes regardless of fold change requires more than 20 biological replicates.