Thiago Carvalho @cyrilpedia@social.cleverlibre.org

Early years of the Coens' genius composer, Carter Burwell:

"One day, Burwell saw a help-wanted ad in the Times for a computer programmer at Cold Spring Harbor Laboratory, a nonprofit research institution whose director, James D. Watson, had shared the Nobel Prize in 1962 for discovering the structure of DNA. Burwell wrote a jokey letter in which he said that, although he had none of the required skills, he would cost less to employ than someone with a Ph.D. would. Surprisingly, the letter got him the job, and he spent two years as the chief computer scientist on a protein-cataloguing project funded by a grant from the Muscular Dystrophy Association."

cc @richardsever

https://www.newyorker.com/magazine/2022/11/14/the-polymath-film-composer-known-as-the-third-coen-brother

"Here, we have pioneered live-imaging techniques and performed functional interrogation to show that the squid Doryteuthis pealeii utilizes mechanisms during retinal neurogenesis that are hallmarks of vertebrate processes. We find that retinal progenitor cells in the squid undergo nuclear migration until they exit the cell cycle. We identify retinal organization corresponding to progenitor, post-mitotic, and differentiated cells. Finally, we find that Notch signaling may regulate both retinal cell cycle and cell fate."

#DevBio #EvoDevo

https://www.cell.com/current-biology/fulltext/S0960-9822(22)01672-4

Tools for off- the-shelf CARTs:

"Conventional CRISPR-Cas nucleases install sequence-specific DNA double-strand breaks (DSBs), enabling gene knock-out (KO) or targeted transgene knock-in (KI). However, simultaneous DSBs provoke a high rate of genomic rearrangements which may impede the safety of the edited cells. Here, we combine a non-viral CRISPR-Cas9 nuclease-assisted KI and Cas9-derived base editing technology for DSB free KOs within a single intervention."

#CARTcells #Immunology #Immunotherapy #crispr #preprint

https://www.biorxiv.org/content/10.1101/2022.11.11.516008v1

@marcdionne So you're saying they set up the pandemic as a smokescreen?

"As with other 19th-century museums, the National Museum was a repository of items plucked, purchased or plundered from Indigenous communities and had presented the people themselves as curiosities, papier-mâché figures in dioramas alongside taxidermied animals. And sometimes worse. The biggest draw of an 1882 exhibition were living members of the Botocudo and Xerente tribes on display for the entertainment of visitors."

https://www.nytimes.com/2022/11/09/magazine/brazil-national-museum-indigenous.html

This one I'd never heard of before:

'A new syndrome that is clinically like poliomyelitis was recognized in children in 2014, and it occurred in the United States in even-numbered years before Covid. This syndrome has subsequently been seen in many countries around the world and has been associated with enterovirus D68.'

#Polio #Vaccine #Virology

https://www.nejm.org/doi/full/10.1056/NEJMp2212115?query=featured_home

"Our previous work established that Aedes (Ae.) aegypti larvae reared in microbe-rich environmental water are less susceptible to ZIKV as adults compared to cohorts reared in microbe-deficient laboratory tap water. Here, we explain the association by identifying a mechanism by which environment-derived microbes reduce susceptibility of Ae. aegypti for ZIKV."

#Zika #Aedes #Microbiome # Virology #preprint

https://www.biorxiv.org/content/10.1101/2022.11.10.516021v1